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Preliminary values and standard operating procedures for determining fecal pollution sources in Utah watersheds


Authors: Natasha Griffin · Rebecca Lee
Owners: iUTAH Data Manager · Natasha Griffin
Resource type:Composite Resource
Created:Aug 25, 2017 at 8:26 p.m.
Last updated: Oct 21, 2017 at 1:33 a.m. by Natasha Griffin

Abstract

We collected water samples from the Logan River, Red Butte Creek, and Provo River in Utah in June 2016 to assess the amount of human, ruminant, and dog fecal contamination in these watersheds. Samples were collected in sterile 1L bottles at sites where the iUTAH NSF EPSCoR program has installed aquatic sensor stations along a mountain-to-urban gradient in each watershed. After collection, we filtered samples through 0.2um PES filters, extracted the sample DNA using a MoBio PowerSoil DNA extraction kit, and analyzed the samples via qPCR.

By identifying the major sources of fecal pollution along urbanization gradients in Utah, we hope to inform efforts to manage fecal pollution in Utah's watersheds. In addition to creating generating raw qPCR values that show the relative contributions of each type (human, ruminant, and dog) to fecal pollution in the rivers sampled, we selected primer sets that we hope will be adopted as standard in future fecal pollution assessment efforts. We also created a standard operating procedure for the creation of qPCR standards, which will allow future researchers to determine total numbers of fecal indicator bacteria present in water samples.

Funded by the iUTAH NSF EPSCoR program (National Science Foundation, iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability, NSF Award Number 1208732) and conducted at Brigham Young University.

Subject Keywords

Microbial source tracking,qPCR,Utah,Provo River,Fecal pollution,Logan River,Red Butte Creek

How to cite

Griffin, N., R. Lee (2017). Preliminary values and standard operating procedures for determining fecal pollution sources in Utah watersheds, HydroShare, http://www.hydroshare.org/resource/2d35730a34c94d91b17f9929807fbf4f

This resource is shared under the Creative Commons Attribution CC BY.

 http://creativecommons.org/licenses/by/4.0/
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Authors

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Name Organization Address Phone Author Identifiers
Natasha Griffin Brigham Young University
Rebecca Lee Brigham Young University

Contributors

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Name Organization Address Phone Author Identifiers
iUTAH Data Manager iUTAH Utah, US

Credits

This resource was created using funding from the following sources:
Agency Name Award Title Award Number
National Science Foundation iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability 1208732

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