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Authors: Erin Jones · Zach Aanderud
Owners: iUTAH Data Manager · Erin Jones
Resource type:Generic
Created:July 27, 2016, 12:36 a.m.
Last updated: July 31, 2017, 6:35 p.m. by Erin Jones

Abstract

This dataset contains .txt (tab delimited) files of OTU (operational taxonomic units) classifications for bacteria at iUTAH GAMUT sites in three watersheds in September 2014. Briefly, water column samples were filtered on 0.2 um membrane filters, which were dissolved and processed with MOBIO Power Soil DNA extraction kits. We used bacterial specific PCR-amplified 16S rDNA and 454-pyrosequencing, and then MOTHUR to generate OTU classifications.

Subject

metagenomics,microbial ecology,water quality,Rank abundance,iUTAH

How to cite

Jones, E., Z. Aanderud (2017). Aquatic GAMUT Bacterial Community Target Metagenomics-454 Pyro, HydroShare, http://www.hydroshare.org/resource/48fc6871c51d436b83000a8d29ddb702

This resource is shared under the Creative Commons Attribution CC BY.

 http://creativecommons.org/licenses/by/4.0/
CC-BY

Sharing status:

  • Public Resource  Public
  • Sharable Resource  Shareable

Coverage

Spatial:

 Coordinate System/Geographic Projection:  WGS 84 EPSG:4326
 Coordinate Units:  Decimal degrees
Place/Area Name: Multiple Watersheds

North Latitude
41.9615°
East Longitude
-110.8809°
South Latitude
40.4010°
West Longitude
-111.9795°

Temporal:

 Start Date:
 End Date:

Content

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Authors

The people or organizations that created the intellectual content of the resource.

Name Organization Address Phone
Erin Jones Brigham Young University
Zach Aanderud Brigham Young University 8014224220
Name Value
Observed Variables Rank abundance
Variable Description Relative abundance of bacterial community taxa in water column
Data Processing Method Sequences have been processed using MOTHUR (Schloss et al. 2009).
Data Collection Method Streamwater aseptically vacuum filtered in the field or lab using autoclaved Nalgene filter cups and 47 mm 0.2 um membrane filters, were stored on dry ice or liquid nitrogen until extracted with MoBIO PowerMax Soil DNA kits. PCR-amplified 16S rDNA samples were sequenced using BYU’s DNA Sequencing Center (454-Pyrosequencing or Hi-Seq).

Credits

This resource was created using funding from the following sources:
Agency Name Award Title Award Number
National Science Foundation iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability 1208732

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Comments

Erin Jones 3 months, 3 weeks ago

Illumina Hi-Seq samples from September 2014-October 2015 are also available on HydroShare.

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