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Biofilm response to nutrient enrichment in urbanizing streams: nutrient diffusing substrate bioassay

Authors: Elizabeth Ogata · Michelle Baker
Owners: iUTAH Data Manager · Elizabeth Ogata
Resource type:Generic
Created:Jul 10, 2016 at 2:43 a.m.
Last updated: Jul 31, 2017 at 12:06 p.m. by iUTAH Data Manager


This dataset contains the results of a nutrient limitation study conducted in the Logan River, the Middle Provo River, Red Butte Creek and the Jordan River. We measured the response of stream biofilms to nitrogen and phosphorus enrichment with nutrient diffusing substrates. Biofilm response was measured as chlorophyll a and ash-free dry mass. The mean, standard deviation, and sample size of biofilm responses to nutrient enrichment treatments are included in this dataset. The dataset also includes the concentration of total nitrogen, total phosphorus, NH4-N, NO3-N, and SRP in stream water samples collected from each site.


ash-free dry mass,bioassay,chlorophyll,nitrogen,nutrient limitation,nutrients,phosphorus,stream biofilms,water quality,Logan River,Red Butte Creek,Middle Provo River,Jordan River,chlorophyll a,iUTAH

How to cite

Ogata, E., M. Baker (2017). Biofilm response to nutrient enrichment in urbanizing streams: nutrient diffusing substrate bioassay, HydroShare, http://www.hydroshare.org/resource/6612b5beff044810b83d56c4db451a85

This resource is shared under the Creative Commons Attribution CC BY.


Sharing status:

  • Public Resource  Public
  • Sharable Resource  Shareable



 Coordinate System/Geographic Projection:  WGS 84 EPSG:4326
 Coordinate Units:  Decimal degrees
North Latitude
East Longitude
South Latitude
West Longitude


 Start Date:
 End Date:


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The people or organizations that created the intellectual content of the resource.

Name Organization Address Phone Author Identifiers
Elizabeth Ogata Utah State University Utah, US
Michelle Baker Utah State University UT, US
Extended Metadata
Name Value
Observed Variables chlorophyll a, ash-free dry mass, total nitrogen, total phosphorus, NH4-N, NO3-N, SRP-P
Variable Description biofilm biomass and stream nutrient concentrations
Data Processing Method For nutrient concentrations below the detection limit, we report half of the method detection limit (method detection limits: 0.0175 mg/L for TN, 0.003 mg/L for TP, 0.005 mg/L for NH4-N, 0.002 mg/L for NO3-N, and 0.002 mg/L for SRP). Data identified as outliers (Tukey method) were excluded.
Data Collection Method Nutrient diffusing substrates (NDS) were made by filling 1-oz plastic cups with agar enriched with treatments of either: no nutrients (Control), nitrogen (+N; 0.5 M NH4Cl), phosphorus (+P; 0.5M KH2PO4), or nitrogen and phosphorus at a series of concentrations (N+P; 0.1, 0.25, 0.5, 1.0 M NH4Cl + KH2PO4). After NDS were incubated in the stream for 14-18 days, the response of stream biofilms to NDS treatments was measured as chlorophyll a and ash-free dry mass.


This resource was created using funding from the following sources:
Agency Name Award Title Award Number
National Science Foundation iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability 1208732


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