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Stream biofilm diphenhydramine dose-response experiment

Authors: Elizabeth Ogata · Donald Long · Michelle Baker · Zachary Aanderud · Emma Rosi
Owners: Elizabeth Ogata · Michelle Baker · iUTAH Data Manager
Resource type:Generic
Created:Oct 01, 2017 at 2:53 p.m.
Last updated: Jul 05, 2018 at 6:57 p.m. by Elizabeth Ogata


This resource contains the results of a diphenhydramine dose-response experiment conducted on stream biofilms at a site on the Logan River in northern Utah. We grew stream biofilms on inorganic (fritted glass disc) and organic (cellulose sponge) substrates in the river for 20 days. The biofilm-colonized substrates were then placed as caps on contaminant exposure substrates (CES), 1-oz plastic cups filled with agar amended with diphenhydramine at a series of concentrations (control, 0.5 mM, 0.75 mM, 1.25m M, 2.5 mM, 15 mM). CES were then deployed in the river for 20 days.

At the end of the CES deployment, the biofilm-colonized substrates were used to perform a series of in-stream incubations. We measured respiration and productivity using a modified light-dark bottle incubation method and nitrogen fixation rates using an acetylene reduction assay. We measured biofilm biomass (chlorophyll a, ash-free dry mass) and calculated Autotrophic Index values (calculated as chlorophyll a concentration divided by ash-free dry mass). The CSV file “biomass_function_diphenhydramine” contains summary statistics (mean, standard deviation, count) of respiration rates, gross primary production rates, nitrogen fixation rates, chlorophyll a concentrations, ash-free dry mass, and Autotrophic Index values of biofilms on each diphenhydramine treatment and substrate type. The Word document “methods_diphenhydramine” describes the analytical methods used to measure chlorophyll and ash-free dry mass.

We characterized light availability and nutrient concentrations at the study site. The Word document “methods_diphenhydramine” describes the methods used to measure site characteristics and the analytical methods used to measure nutrient concentrations. The CSV file “site_characteristics_diphenhydramine” contains percent canopy openness, transmitted PAR, transmitted solar shortwave radiation, total nitrogen, total phosphorus, ammonium, nitrate, soluble reactive phosphorus, dissolved total iron, and dissolved ferrous iron concentrations.

Subject Keywords

chlorophyll,respiration,stream biofilm,diphenhydramine,dose-response,nitrogen fixation,contaminant exposure substrate,ash-free dry mass,Logan River,iUTAH,gross primary production

How to cite

Ogata, E., D. Long, M. Baker, Z. Aanderud, E. Rosi (2018). Stream biofilm diphenhydramine dose-response experiment, HydroShare, http://www.hydroshare.org/resource/7b551c0d6ef147b1bc2a006b2b898e1b

This resource is shared under the Creative Commons Attribution CC BY.


Sharing status:

  • Public Resource  Public
  • Sharable Resource  Shareable



 Coordinate System/Geographic Projection:  WGS 84 EPSG:4326
 Coordinate Units:  Decimal degrees
Place/Area Name: Logan River



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The people or organizations that created the intellectual content of the resource.

Name Organization Address Phone Author Identifiers
Elizabeth Ogata Utah State University
Donald Long Southern Utah University
Michelle Baker Utah State University UT, US
Zachary Aanderud Brigham Young University
Emma Rosi Cary Institute of Ecosystem Studies
Extended Metadata
Name Value
Observed variables in "biomass_function_diphenhydramine" CSV file gross primary production (mg O2/m2/hr), respiration (mg O2/m2/hr), nitrogen fixation rate (ug N2/m2/hr), chlorophyll a concentration (chl; mg/m2), ash-free dry mass (g/m2), Autotrophic Index (calculated as ash-free dry mass (mg/m2) divided by chlorophyll a concentration (mg/m2))
Observed variables in "site_characteristics_diphenhydramine" CSV file total nitrogen (mg N/L), total phosphorus (mg P/L), ammonium (mg NH4-N/L), nitrate (mg NO3-N+NO2-N/L), soluble reactive phosphorus (mg SRP-P/L), total dissolved iron (ug/L), dissolved ferrous iron (ug/L), percent canopy openness, transmitted photosynthetically active radiation (umols/m2/day), transmitted solar shortwave radiation (W/m2/day)


This resource was created using funding from the following sources:
Agency Name Award Title Award Number
National Science Foundation iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability 1208732


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