Aquatic GAMUT Eukaryote Metagenomics

Authors:	Erin Jones · Zach Aanderud
Owners:	iUTAH Data Manager · Erin Jones
Resource type:	Generic
Created:	July 31, 2017, 6:10 p.m.
Last updated:	July 31, 2017, 6:19 p.m. by Erin Jones

Abstract

This dataset contains .txt (tab delimited) files of OTU (operational taxonomic units) classifications for eukaryotes at iUTAH GAMUT sites in three watersheds in May of 2015. Briefly, water column samples were filtered on 0.2 um membrane filters, which were dissolved and processed with MOBIO Power Soil DNA extraction kits. We Illumina Hi-Seq and then MG-RAST to generate OTU classifications.

How to cite

Jones, E., Z. Aanderud (2017). Aquatic GAMUT Eukaryote Metagenomics, HydroShare, http://www.hydroshare.org/resource/a80e4fa33a644691a29ab80b8cfc1aae

This resource is shared under the Creative Commons Attribution CC BY.

http://creativecommons.org/licenses/by/4.0/

Coverage

Spatial:

Coordinate System/Geographic Projection:	WGS 84 EPSG:4326
Coordinate Units:	Decimal degrees

Place/Area Name: Multiple Watersheds

North Latitude

41.9615°

East Longitude

-110.8809°

South Latitude

40.4010°

West Longitude

-111.9795°

Temporal:

Start Date:
End Date:

Authors

The people or organizations that created the intellectual content of the resource.

Name	Organization	Address	Phone
Erin Jones	Brigham Young University
Zach Aanderud	Brigham Young University		8014224220

Sources

Derived From:

http://www.hydroshare.org/resource/48fc6871c51d436b83000a8d29ddb702

Name	Value
Observed Variables	Rank abundance
Variable Description	Relative abundance of bacterial community taxa in water column
Data Processing Method	Sequences have been processed using MOTHUR (Schloss et al. 2009).
Data Collection Method	Streamwater aseptically vacuum filtered in the field or lab using autoclaved Nalgene filter cups and 47 mm 0.2 um membrane filters, were stored on dry ice or liquid nitrogen until extracted with MoBIO PowerMax Soil DNA kits. PCR-amplified 16S rDNA samples were sequenced using BYU’s DNA Sequencing Center (454-Pyrosequencing or Hi-Seq).

Credits

This resource was created using funding from the following sources:

Agency Name	Award Title	Award Number
National Science Foundation	iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability	1208732

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