Aquatic GAMUT Eukaryote Metagenomics
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| Resource type: | Composite Resource | |
| Storage: | The size of this resource is 1.7 MB | |
| Created: | Jul 31, 2017 at 6:10 p.m. | |
| Last updated: | Jun 19, 2018 at 11:10 p.m.
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| Sharing Status: | Public |
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| Downloads: | 158 |
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Abstract
This dataset contains a .biom file of OTU (operational taxonomic units) classifications for eukaryotes at iUTAH GAMUT sites in three watersheds in May of 2015. Briefly, water column samples were filtered on 0.2 um membrane filters, which were dissolved and processed with MOBIO Power Soil DNA extraction kits. We sequenced 18S region amplicons using Illumina Hi-Seq and then a modified MOTHUR protocol (Silva v. 132 reference file, command codes in mothureukaryotes.txt) to generate OTU classifications. Sample metadata (including site information and sample chemistry from grab samples or sensor data) is in design.csv.
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Resource Level Coverage
Spatial
Coordinate System/Geographic Projection:
WGS 84 EPSG:4326
Coordinate Units:
Decimal degrees
Place/Area Name:
Multiple Watersheds
North Latitude
41.9615°
East Longitude
-110.8809°
South Latitude
40.4010°
West Longitude
-111.9795°
Temporal
| Start Date: | |
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| End Date: |
Content
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Additional Metadata
| Name | Value |
|---|---|
| Observed Variables | Rank abundance |
| Variable Description | Relative abundance of bacterial community taxa in water column |
| Data Collection Method | Streamwater aseptically vacuum filtered in the field or lab using autoclaved Nalgene filter cups and 47 mm 0.2 um membrane filters, were stored on dry ice or liquid nitrogen until extracted with MoBIO PowerMax Soil DNA kits. PCR-amplified 16S rDNA samples were sequenced using BYU’s DNA Sequencing Center (454-Pyrosequencing or Hi-Seq). |
| Data Processing Method | Sequences have been processed using MOTHUR (Schloss et al. 2009). |
References
Sources
| Derived From: | http://www.hydroshare.org/resource/48fc6871c51d436b83000a8d29ddb702 |
Credits
Funding Agencies
This resource was created using funding from the following sources:
| Agency Name | Award Title | Award Number |
|---|---|---|
| National Science Foundation | iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability | 1208732 |
How to Cite
| Jones, E., Z. Aanderud (2018). Aquatic GAMUT Eukaryote Metagenomics, HydroShare, http://www.hydroshare.org/resource/a80e4fa33a644691a29ab80b8cfc1aae |
This resource is shared under the Creative Commons Attribution CC BY.
http://creativecommons.org/licenses/by/4.0/
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