Aquatic GAMUT Eukaryote Metagenomics
|Authors:||Erin Jones Zach Aanderud|
|Storage:||The size of this resource is 1.7 MB|
|Created:||Jul 31, 2017 at 6:10 p.m.|
|Last updated:|| Jun 19, 2018 at 11:10 p.m.
|Citation:||See how to cite this resource|
This dataset contains a .biom file of OTU (operational taxonomic units) classifications for eukaryotes at iUTAH GAMUT sites in three watersheds in May of 2015. Briefly, water column samples were filtered on 0.2 um membrane filters, which were dissolved and processed with MOBIO Power Soil DNA extraction kits. We sequenced 18S region amplicons using Illumina Hi-Seq and then a modified MOTHUR protocol (Silva v. 132 reference file, command codes in mothureukaryotes.txt) to generate OTU classifications. Sample metadata (including site information and sample chemistry from grab samples or sensor data) is in design.csv.
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Resource Level Coverage
|Observed Variables||Rank abundance|
|Variable Description||Relative abundance of bacterial community taxa in water column|
|Data Processing Method||Sequences have been processed using MOTHUR (Schloss et al. 2009).|
|Data Collection Method||Streamwater aseptically vacuum filtered in the field or lab using autoclaved Nalgene filter cups and 47 mm 0.2 um membrane filters, were stored on dry ice or liquid nitrogen until extracted with MoBIO PowerMax Soil DNA kits. PCR-amplified 16S rDNA samples were sequenced using BYU’s DNA Sequencing Center (454-Pyrosequencing or Hi-Seq).|
This resource was created using funding from the following sources:
|Agency Name||Award Title||Award Number|
|National Science Foundation||iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability||1208732|
How to Cite
This resource is shared under the Creative Commons Attribution CC BY.http://creativecommons.org/licenses/by/4.0/
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