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Aquatic GAMUT Eukaryote Metagenomics

Authors: Erin Jones · Zach Aanderud
Owners: Erin Jones · iUTAH Data Manager
Resource type:Generic
Created:Jul 31, 2017 at 6:10 p.m.
Last updated: Jun 13, 2018 at 6:17 p.m. by Erin Jones

Abstract

This dataset contains a .biom file of OTU (operational taxonomic units) classifications for eukaryotes at iUTAH GAMUT sites in three watersheds in May of 2015. Briefly, water column samples were filtered on 0.2 um membrane filters, which were dissolved and processed with MOBIO Power Soil DNA extraction kits. We sequenced 18S region amplicons using Illumina Hi-Seq and then a modified MOTHUR protocol (Silva v. 132 reference file, command codes in mothureukaryotes.txt) to generate OTU classifications. Sample metadata (including site information and sample chemistry from grab samples or sensor data) is in design.csv.

Subject

metagenomics,microbial ecology,water quality,Rank abundance,iUTAH

How to cite

Jones, E., Z. Aanderud (2018). Aquatic GAMUT Eukaryote Metagenomics, HydroShare, http://www.hydroshare.org/resource/a80e4fa33a644691a29ab80b8cfc1aae

This resource is shared under the Creative Commons Attribution CC BY.

  http://creativecommons.org/licenses/by/4.0/
CC-BY

Sharing status:

  • Public Resource  Public
  • Sharable Resource  Shareable

Coverage

Spatial:

 Coordinate System/Geographic Projection:  WGS 84 EPSG:4326
 Coordinate Units:  Decimal degrees
Place/Area Name: Multiple Watersheds

North Latitude
41.9615°
East Longitude
-110.8809°
South Latitude
40.4010°
West Longitude
-111.9795°

Temporal:

 Start Date:
 End Date:

Content

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Authors

The people or organizations that created the intellectual content of the resource.

Name Organization Address Phone Author Identifiers
Erin Jones Brigham Young University
Zach Aanderud Brigham Young University 8014224220
Extended Metadata
Name Value
Observed Variables Rank abundance
Variable Description Relative abundance of bacterial community taxa in water column
Data Processing Method Sequences have been processed using MOTHUR (Schloss et al. 2009).
Data Collection Method Streamwater aseptically vacuum filtered in the field or lab using autoclaved Nalgene filter cups and 47 mm 0.2 um membrane filters, were stored on dry ice or liquid nitrogen until extracted with MoBIO PowerMax Soil DNA kits. PCR-amplified 16S rDNA samples were sequenced using BYU’s DNA Sequencing Center (454-Pyrosequencing or Hi-Seq).

Credits

This resource was created using funding from the following sources:
Agency Name Award Title Award Number
National Science Foundation iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability 1208732

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