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Created: Apr 22, 2024 at 2:07 a.m.
Last updated: Apr 22, 2024 at 2:33 a.m.
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This study was conducted in the South Fork of King’s Creek at Konza Prairie Biological Station, one of the most well-studied prairie streams in the world. At the USGS gage located on the mainstem (06879560; est. 1979), Kings Creek is a 5th order intermittent stream draining 1059-ha of tallgrass prairie in the Kansas Flint Hills.
This synoptic survey was designed in support of the sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. During June, July, and August 2021, a field team co-collected datasets characterizing the hydrology, biogeochemistry, and ecology across 50 locations within a sub-drainage of the South Fork of Kings’ Creek. The 50 sites were selected to balance multiple competing priorities: (i) strategically targeting existing monitoring infrastructure with long-term data (n=14); (ii) including sites near several known springs and tributary junctions (n=9); and (iii) including a range of drainage area and topographic wetness index (TWI) values (n=27), both of which have been correlated with flow permanence elsewhere. Briefly, the sites selected based on drainage area and TWI were chosen by binning drainage area into 10 bins and then binning TWI into quintiles within each drainage area bin. We then randomly selected a point in each bin after accounting for points selected based on existing infrastructure, springs, and tributaries;, and enforcing a minimum spacing of 100 m between locations. We then made minor adjustments to points to account for field conditions, for instance adjusting locations with respect to a road crossing. For a detailed description of the site selection process, please see (Swenson et al., 2023).

Subject Keywords



Coordinate System/Geographic Projection:
WGS 84 EPSG:4326
Coordinate Units:
Decimal degrees
Place/Area Name:
Kings Creek, KS; Youngmeyer Ranch, KS; Oka’Yanahli National Preserve, OK
North Latitude
East Longitude
South Latitude
West Longitude


Start Date:
End Date:



Funding Agencies

This resource was created using funding from the following sources:
Agency Name Award Title Award Number
NSF OIA AIMS 2019603

How to Cite

Zeglin, L., B. A. Nave (2024). AIMS_GP_approach3_QPCR, HydroShare,

This resource is shared under the Creative Commons Attribution CC BY.


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