ABSTRACT:

This study was conducted in the Talladega research watershed (outlet location: 33.76219799, -85.59550775) in the Talladega National Forest (Cleburne County, AL, USA). The watershed drains a non-perennial unnamed tributary of Pendergrass Creek, and contains 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. Located near Anniston, AL, the watershed spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr. These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 7 October 2021 and 30 January 2023, grab samples for Total Suspended Solids (TSS, mg/L) and seston Ash Free Dry Mass (AFDM, mg/L) were collected at the watershed outlet (TLM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), seasonally at seven distributed, long term monitoring sites (AIMS Approach 2), and during a large synoptic sampling efforts concentrated across the watershed (AIMS Approach 3). Additional "stretch" samples were collected for TSS and AFDM at a tributary (TLC01) and downstream perennial monitoring site (TLP01), which are included in a separate file from the core TSS data from the Talladega research watershed. To measure TSS and AFDM concentrations, we collected 2- 3 replicates of unfiltered stream water in clean 1L Nalgene bottles after triple rinsing in the field, stored on ice, and brought to the lab for filtering. We brought samples back to the lab and filtered samples in 100mL batches through pre-ashed (550℃ for one hour) and pre-weighed 47 mm Whatman GF/F filters. Once the sample was filtered, the filters were placed in a drying oven for at least 48 hours and weighed. TSS was calculated as the (dry weight (filter + tin + sample) - dry weight (filter + tin)) / volume filtered (L). Ash free dry mass (AFDM) was then calculated as (dry weight (filter + tin + sample) - ashed weight (filter + tin + sample)) / volume filtered (L).

ABSTRACT:

This study was conducted in the Shambley Creek research watershed (outlet location: 32.98410915, -88.01334337) on privately owned property in Greene County (AL, USA). The watershed drains a non-perennial unnamed tributary to Shambley Creek, and contains 0.70 km^2 of coniferous forest managed for silviculture in the East Gulf Coastal Plain physiographic section. Located near Eutaw, AL, the watershed spans an elevation range from 63 to 94 m above sea level, and is a tributary to the Sipsey River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 7.3°C and 27.4°C respectively, and mean annual precipitation of 1,350 mm/yr. These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 14 October 2021 and 7 February 2023, grab samples for Total Suspended Solids (TSS, mg/L) and seston Ash Free Dry Mass (AFDM, mg/L) were collected at the watershed outlet (WHM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1) and seasonally at seven distributed, long term monitoring sites (AIMS Approach 2). Additional "stretch" samples were collected for TSS and AFDM at a tributary (WHB01) and downstream perennial monitoring site (WHP01), which are included in a separate file from the core TSS data from the Shambley Creek research watershed. To measure TSS and AFDM concentrations, we collected 2- 3 replicates of unfiltered stream water in clean 1L Nalgene bottles after triple rinsing in the field, stored on ice, and brought to the lab for filtering. We brought samples back to the lab and filtered samples in 100mL batches through pre-ashed (550℃ for one hour) and pre-weighed 47 mm Whatman GF/F filters. Once the sample was filtered, the filters were placed in a drying oven for at least 48 hours and weighed. TSS was calculated as the (dry weight (filter + tin + sample) - dry weight (filter + tin)) / volume filtered (L). Ash free dry mass (AFDM) was then calculated as (dry weight (filter + tin + sample) - ashed weight (filter + tin + sample)) / volume filtered (L).

ABSTRACT:

This study was conducted in the Paint Rock research watershed (outlet location: 34.96861724, -86.16501705) on privately owned property in Jackson County (AL, USA). The watershed drains a non-perennial unnamed tributary to Burks Creek, and contains 2.97 km^2 of deciduous forest in the Cumberland Plateau physiographic section. Located near Estillfork, AL, the watershed spans an elevation range from 211 to 550 m above sea level, and is a tributary to the Paint Rock River (within the larger Tennessee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 4.4°C and 25.4°C respectively, and mean annual precipitation of 1,390 mm/yr. These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 13 October 2021 and 13 February 2023, grab samples for Total Suspended Solids (TSS, mg/L) and seston Ash Free Dry Mass (AFDM, mg/L) were collected at the watershed outlet (PRM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1) and seasonally at seven distributed, long term monitoring sites (AIMS Approach 2). Additional "stretch" samples were collected for TSS and AFDM at a tributary (PRB02) and downstream perennial monitoring site (PRP01), which are included in a separate file from the core TSS data from the Paint Rock research watershed. To measure TSS and AFDM concentrations, we collected 2- 3 replicates of unfiltered stream water in clean 1L Nalgene bottles after triple rinsing in the field, stored on ice, and brought to the lab for filtering. We brought samples back to the lab and filtered samples in 100mL batches through pre-ashed (550℃ for one hour) and pre-weighed 47 mm Whatman GF/F filters. Once the sample was filtered, the filters were placed in a drying oven for at least 48 hours and weighed. TSS was calculated as the (dry weight (filter + tin + sample) - dry weight (filter + tin)) / volume filtered (L). Ash free dry mass (AFDM) was then calculated as (dry weight (filter + tin + sample) - ashed weight (filter + tin + sample)) / volume filtered (L).

ABSTRACT:

These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. This study was conducted in the Talladega research watershed (outlet location: 33.76219799, -85.59550775) in the Talladega National Forest (Cleburne County, AL, USA). The watershed drains a non-perennial unnamed tributary of Pendergrass Creek, and contains 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. Located near Anniston, AL, the watershed spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr. Between 7 October 2021 and 4 October 2024, grab samples for Dissolved Organic Carbon concentration (DOC, mg/L) collected at the watershed outlet (TLM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), seasonally at seven distributed, long term monitoring sites (AIMS Approach 2), and during a large synoptic sampling efforts concentrated across the watershed (AIMS Approach 3). DOC samples were filtered through pre-ashed, 0.7-micron glass fiber filters (Whatman GF/F) into acid-washed, 60-mL amber high-density polyethylene (HDPE) bottles. DOC samples were preserved by acidifying to a ph = 2 using concentrated HCl and refrigerated until analysis within 28 days of collection. DOC concentrations were determined as non-purgeable organic carbon (NPOC) using a total organic carbon analyzer (Schimadzu TOC-V).

ABSTRACT:

These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. This study was conducted in the Shambley Creek research watershed (outlet location: 32.98410915, -88.01334337) on privately owned property in Greene County (AL, USA). The watershed drains a non-perennial unnamed tributary to Shambley Creek, and contains 0.70 km^2 of coniferous forest managed for silviculture in the East Gulf Coastal Plain physiographic section. Located near Eutaw, AL, the watershed spans an elevation range from 63 to 94 m above sea level, and is a tributary to the Sipsey River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 7.3°C and 27.4°C respectively, and mean annual precipitation of 1,350 mm/yr. Between 14 October 2021 and 7 July 2024, grab samples for Dissolved Organic Carbon concentration (DOC, mg/L) collected at the watershed outlet (WHM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1) and seasonally at seven distributed, long term monitoring sites (AIMS Approach 2). DOC samples were filtered through pre-ashed, 0.7-micron glass fiber filters (Whatman GF/F) into acid-washed, 60-mL amber high-density polyethylene (HDPE) bottles. DOC samples were preserved by acidifying to a ph = 2 using concentrated HCl and refrigerated until analysis within 28 days of collection. DOC concentrations were determined as non-purgeable organic carbon (NPOC) using a total organic carbon analyzer (Schimadzu TOC-V).

ABSTRACT:

This study was conducted in the Paint Rock research watershed (outlet location: 34.96861724, -86.16501705) on privately owned property in Jackson County (AL, USA). The watershed drains a non-perennial unnamed tributary to Burks Creek, and contains 2.97 km^2 of deciduous forest in the Cumberland Plateau physiographic section. Located near Estillfork, AL, the watershed spans an elevation range from 211 to 550 m above sea level, and is a tributary to the Paint Rock River (within the larger Tennessee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 4.4°C and 25.4°C respectively, and mean annual precipitation of 1,390 mm/yr. These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 13 October 2021 and 27 June 2024, grab samples for Dissolved Organic Carbon concentration (DOC, mg/L) collected at the watershed outlet (PRM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), seasonally at seven distributed, long term monitoring sites (AIMS Approach 2), and during a large synoptic sampling efforts concentrated across the watershed (AIMS Approach 3). DOC samples were filtered through pre-ashed, 0.7-micron glass fiber filters (Whatman GF/F) into acid-washed, 60-mL amber high-density polyethylene (HDPE) bottles. DOC samples were preserved by acidifying to a ph = 2 using concentrated HCl and refrigerated until analysis within 28 days of collection. DOC concentrations were determined as non-purgeable organic carbon (NPOC) using a total organic carbon analyzer (Schimadzu TOC-V).

ABSTRACT:

This resource contains the results from a set of whole-stream isotopically-label dissolved organic carbon (13C-DOC) addition experiments conducted in August 2019 to directly trace the uptake and mineralization of DOC into the stream DIC pool. These experiments were conducted in Blaine Creek, an open-canopy, agricultural, spring-fed stream located a the Montana State University Northwestern Agricultural Research Center in Creston, Montana, USA. Between 8 and 15 August 2019, we conducted three separate pulse releases of NaCl (used as a conservative solute tracer) and a reactive 13C-labeled DOC substrate, either 13C-labeled glucose on 8 and 15 August 2019 (as 3 grams of D-glucose dissolved in 100 mL of deionized water) or 13C-labeled leaf leachate on 9 August 2019. The 13C-labeled leaf leachate was prepared following Kaplan et al. (2008) using uniformly labeled 13C red fescue in deionized water. During each pulse release, we sampled for DOC, pH, alkalinity and CO2 at two sampling stations 41 (denoted as "upstream") and 61 meters (denoted as "downstream") downstream of the addition site, respectively. We also deployed sensors to collect dissolved O2 and temperature (PME miniDOT) and dissolved CO2 (Eosense eosGP) sensors at 10 minute intervals throughout the study period to estimate CO2 emission fluxes and daily rates of of ecosystem metabolism (i.e., gross primary production and ecosystem respiration). The file "blaine_65_oxy.csv" contains the 10-minute interval temperature and dissolved O2 sensor data from the downstream sampling station, which was used to estimate daily rates of metabolism.

The file "pco2_blaine.csv" contains the 10-minute interval temperature and dissolved CO2 data from both the upstream (denoted as "white" in column names) and downstream (denoted as "bela" in column names) sampling stations. The file "site_data.csv" contains relevant information to each pulse release such as reach length (in meters), mean wetted width (in meters), conservative and reactive tracer masses (in grams for "salt_mass" and "sugar mass", in milligrams for "doc_mass_13C_mg"), alkalinity, travel time between addition and sampling station (in minutes), as well as velocity (v.ms, in meters per second) and discharge (Qest.Ls, in liters per second) estimated from the conservative tracer during each addition.

For samples collected throughout each pulse release, we quantified 13-CO2 from headspace equilibrated samples immediately following the experiments using a Cavity Ring-Down Spectrometer (CRDS; Picarro), alkalinity via end-point titration, and DOC concentrations and del13C-DOC using a TOC analyzer (Aurora 1030, OI Analytical) coupled to a Picarro CRDS. The folder "DOC_runs" contains individual CSV files for each pulse release with the DOC concentration ("DOC_conc", in milligrams per liter), del13C-DOC ("Delta 13C"), and alkalinity (in meq/L) for each sampling timepoint. The folder "picarro_runs" contains individual CSV files for each pulse release with the CO2 and CH4 concentration ("CO2" and "CH4" in ppm), del13C-CO2 ("delCO2"), and del13C-CH4 ("delCH4") values for each sampling timepoint.

All file and column names are preserved to ensure coding and modeling workflows associated with this resource function, which can be found at https://github.com/robohall/DOC_uptake

References

Kaplan, L. A., T. N. Weigner, J. D. Newbold, P. H. Ostrom, and H. Gandhi. 2008. Untangling the complex issue of dissolved organic carbon uptake: a stable isotope approach. Freshwater Biology, 53:855–864.

ABSTRACT:

This study was conducted in the Talladega research watershed (outlet location: 33.76219799, -85.59550775) in the Talladega National Forest (Cleburne County, AL, USA). The watershed drains a non-perennial unnamed tributary of Pendergrass Creek, and contains 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. Located near Anniston, AL, the watershed spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr. These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 7 October 2021 and 7 October 2024, we conducted pulse additions of NaCl (i.e., "salt slugs") to estimate discharge (Q; liters per second) and velocity (v; meters per second) at the watershed outlet (TLM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), seasonally at seven distributed, long term monitoring sites (AIMS Approach 2), and during a large synoptic sampling efforts concentrated across the watershed (AIMS Approach 3).

Briefly, discharge and velocity were estimated by adding a known mass of NaCl dissolved in approximately 1 liter of stream water to an upstream "addition" site and measuring changes in conductivity using a Solinst Conductivity Sensor (at 2 second intervals) at a downstream "logging" site. Discharge (in liters per second) was estimated using the mass of salt added and the area under the background conductivity-corrected "breakthrough curve" (e.g., change in conductivity over time at the logging site from the arrival of the salt tracer until conductivity returns to pre-salt slug background levels). Velocity (in meters per second) was estimated using nominal travel time (time for 50% of salt mass to pass the logging site) and the reach length between the addition and logging site. Salt slugs were only conducted if the reach upstream of the sample site was fully connected and flowing continuously for a distance of at least ten wetted widths. For R scripts and individual tracer breakthrough curves used to estimate discharge and travel time, please contact Stephen Plont (plontste@gmail.com).

ABSTRACT:

This study was conducted in the Shambley Creek research watershed (outlet location: 32.98410915, -88.01334337) on privately owned property in Greene County (AL, USA). The watershed drains a non-perennial unnamed tributary to Shambley Creek, and contains 0.70 km^2 of coniferous forest managed for silviculture in the East Gulf Coastal Plain physiographic section. Located near Eutaw, AL, the watershed spans an elevation range from 63 to 94 m above sea level, and is a tributary to the Sipsey River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 7.3°C and 27.4°C respectively, and mean annual precipitation of 1,350 mm/yr. These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 14 October 2021 and 7 May 2024, we conducted pulse additions of NaCl (i.e., "salt slugs") to estimate discharge (Q; liters per second) and velocity (v; meters per second) at the watershed outlet (TLM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1) and seasonally at seven distributed, long term monitoring sites (AIMS Approach 2).

Briefly, discharge and velocity were estimated by adding a known mass of NaCl dissolved in approximately 1 liter of stream water to an upstream "addition" site and measuring changes in conductivity using a Solinst Conductivity Sensor (at 2 second intervals) at a downstream "logging" site. Discharge (in liters per second) was estimated using the mass of salt added and the area under the background conductivity-corrected "breakthrough curve" (e.g., change in conductivity over time at the logging site from the arrival of the salt tracer until conductivity returns to pre-salt slug background levels). Velocity (in meters per second) was estimated using nominal travel time (time for 50% of salt mass to pass the logging site) and the reach length between the addition and logging site. Salt slugs were only conducted if the reach upstream of the sample site was fully connected and flowing continuously for a distance of at least ten wetted widths. For R scripts and individual tracer breakthrough curves used to estimate discharge and travel time, please contact Stephen Plont (plontste@gmail.com)

ABSTRACT:

This study was conducted in the Paint Rock research watershed (outlet location: 34.96861724, -86.16501705) on privately owned property in Jackson County (AL, USA). The watershed drains a non-perennial unnamed tributary to Burks Creek, and contains 2.97 km^2 of deciduous forest in the Cumberland Plateau physiographic section. Located near Estillfork, AL, the watershed spans an elevation range from 211 to 550 m above sea level, and is a tributary to the Paint Rock River (within the larger Tennessee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 4.4°C and 25.4°C respectively, and mean annual precipitation of 1,390 mm/yr. These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 13 October 2021 and 6 June 2024, we conducted pulse additions of NaCl (i.e., "salt slugs") to estimate discharge (Q; liters per second) and velocity (v; meters per second) at the watershed outlet (TLM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1) and seasonally at seven distributed, long term monitoring sites (AIMS Approach 2).

Briefly, discharge and velocity were estimated by adding a known mass of NaCl dissolved in approximately 1 liter of stream water to an upstream "addition" site and measuring changes in conductivity using a Solinst Conductivity Sensor (at 2 second intervals) at a downstream "logging" site. Discharge (in liters per second) was estimated using the mass of salt added and the area under the background conductivity-corrected "breakthrough curve" (e.g., change in conductivity over time at the logging site from the arrival of the salt tracer until conductivity returns to pre-salt slug background levels). Velocity (in meters per second) was estimated using nominal travel time (time for 50% of salt mass to pass the logging site) and the reach length between the addition and logging site. Salt slugs were only conducted if the reach upstream of the sample site was fully connected and flowing continuously for a distance of at least ten wetted widths. For R scripts and individual tracer breakthrough curves used to estimate discharge and travel time, please contact Stephen Plont (plontste@gmail.com)

ABSTRACT:

These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. This study was conducted in the Talladega research watershed (outlet location: 33.76219799, -85.59550775) in the Talladega National Forest (Cleburne County, AL, USA). The watershed drains a non-perennial unnamed tributary of Pendergrass Creek, and contains 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. Located near Anniston, AL, the watershed spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr. Between 29 March 2022 and 4 October 2024, grab samples for cation determination were collected at the watershed outlet (TLM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), seasonally at seven distributed, long term monitoring sites (AIMS Approach 2), and during a large synoptic sampling efforts concentrated across the watershed (AIMS Approach 3). Samples were filtered in the field with a 0.45um PES filter and acidified to 2% nitric acid for preservation until analysis. Immediately prior to analysis, samples were filtered with a 0.2um PES filter. Aqueous concentrations of cations were determined using coupled plasma-atomic emission spectrometry (ICP-AES) (Horiba Ultima 2, Horiba Jobin Yvon) fitted with an AS 500 autosampler. The instrument has a glass concentric nebulizer equipped with a cyclonic chamber. The generator power is 1000 W. The plasma and auxiliary gas flow rate are 12 L/min and 0 L/min. The sheath gas flow rate and nebulizer are 0.2 and 0.8 L/min. Argon pressure is 80 psi. Multi-elemental standards are prepared in 2% nitric acid as well.

Intermediate tab has data from all triplicates; final data shows only triplicate average, st dev, and RSD (relative st dev). RSD (relative standard deviation is included in intermediate tab; red color indicates values above 10%). We recommend that users use what is presented in the final data tab, as not every replicate has been accounted for and the data has been cleaned.

Low standard for each analyte (below): our lab uses this as the conservative detection limit/LOD. Na - 0.5 ppm / Ca - 0.5 ppm / B - 0.02 ppm / Mg - 0.5 ppm / Si - 0.5 ppm / K - 0.2 ppm / Sr - 0.05 ppm. Note: Boron (B) is almost always below our lowest standard. Most of this data is not useable/below our minimum standard.

ABSTRACT:

These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. This study was conducted in the Paint Rock research watershed (outlet location: 34.96861724, -86.16501705) on privately owned property in Jackson County (AL, USA). The watershed drains a non-perennial unnamed tributary to Burks Creek, and contains 2.97 km^2 of deciduous forest in the Cumberland Plateau physiographic section. Located near Estillfork, AL, the watershed spans an elevation range from 211 to 550 m above sea level, and is a tributary to the Paint Rock River (within the larger Tennessee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 4.4°C and 25.4°C respectively, and mean annual precipitation of 1,390 mm/yr. Between 14 March 2022 and 1 August 2024, grab samples for cation determination were collected at the watershed outlet (PRM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), and seasonally at seven distributed, long term monitoring sites across the watershed (AIMS Approach 2). Samples were filtered in the field with a 0.45um PES filter and acidified to 2% nitric acid for preservation until analysis. Immediately prior to analysis, samples were filtered with a 0.2um PES filter. Aqueous concentrations of cations were determined using coupled plasma-atomic emission spectrometry (ICP-AES) (Horiba Ultima 2, Horiba Jobin Yvon) fitted with an AS 500 autosampler. The instrument has a glass concentric nebulizer equipped with a cyclonic chamber. The generator power is 1000 W. The plasma and auxiliary gas flow rate are 12 L/min and 0 L/min. The sheath gas flow rate and nebulizer are 0.2 and 0.8 L/min. Argon pressure is 80 psi. Multi-elemental standards are prepared in 2% nitric acid as well.

Intermediate tab has data from all triplicates; final data shows only triplicate average, st dev, and RSD (relative st dev). RSD (relative standard deviation is included in intermediate tab; red color indicates values above 10%). We recommend that users use what is presented in the final data tab, as not every replicate has been accounted for and the data has been cleaned.

Low standard for each analyte (below): our lab uses this as the conservative detection limit/LOD. Na - 0.5 ppm / Ca - 0.5 ppm / B - 0.02 ppm / Mg - 0.5 ppm / Si - 0.5 ppm / K - 0.2 ppm / Sr - 0.05 ppm. Note: Boron (B) is almost always below our lowest standard. Most of this data is not useable/below our minimum standard.

ABSTRACT:

These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. This study was conducted in the Shambley Creek research watershed (outlet location: 32.98410915, -88.01334337) on privately owned property in Greene County (AL, USA). The watershed drains a non-perennial unnamed tributary to Shambley Creek, and contains 0.70 km^2 of coniferous forest managed for silviculture in the East Gulf Coastal Plain physiographic section. Located near Eutaw, AL, the watershed spans an elevation range from 63 to 94 m above sea level, and is a tributary to the Sipsey River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 7.3°C and 27.4°C respectively, and mean annual precipitation of 1,350 mm/yr. Between 24 March 2022 and 30 July 2024, grab samples for cation determination were collected at the watershed outlet (WHM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), and seasonally at seven distributed, long term monitoring sites across the watershed (AIMS Approach 2). Samples were filtered in the field with a 0.45um PES filter and acidified to 2% nitric acid for preservation until analysis. Immediately prior to analysis, samples were filtered with a 0.2um PES filter. Aqueous concentrations of cations were determined using coupled plasma-atomic emission spectrometry (ICP-AES) (Horiba Ultima 2, Horiba Jobin Yvon) fitted with an AS 500 autosampler. The instrument has a glass concentric nebulizer equipped with a cyclonic chamber. The generator power is 1000 W. The plasma and auxiliary gas flow rate are 12 L/min and 0 L/min. The sheath gas flow rate and nebulizer are 0.2 and 0.8 L/min. Argon pressure is 80 psi. Multi-elemental standards are prepared in 2% nitric acid as well.

Intermediate tab has data from all triplicates; final data shows only triplicate average, st dev, and RSD (relative st dev). RSD (relative standard deviation is included in intermediate tab; red color indicates values above 10%). We recommend that users use what is presented in the final data tab, as not every replicate has been accounted for and the data has been cleaned.

Low standard for each analyte (below): our lab uses this as the conservative detection limit/LOD. Na - 0.5 ppm / Ca - 0.5 ppm / B - 0.02 ppm / Mg - 0.5 ppm / Si - 0.5 ppm / K - 0.2 ppm / Sr - 0.05 ppm. Note: Boron (B) is almost always below our lowest standard. Most of this data is not useable/below our minimum standard.

ABSTRACT:

This study was conducted in the Talladega research watershed (outlet location: 33.76219799, -85.59550775) in the Talladega National Forest (Cleburne County, AL, USA). The watershed drains a non-perennial unnamed tributary of Pendergrass Creek, and contains 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. Located near Anniston, AL, the watershed spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr.

These data were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 24 August 2021 and 15 October 2024, we monitored surface water level at the outlet (TLM01) of the AIMS Talladega watershed at 15 minute intervals using a pressure transducer (Seametrics PT12 vented pressure/temperature sensor from 24 August 2021 to 31 December 2022 and Onset HOBO U20 series unvented sensor from 1 January 2023 to 15 October 2024). Over the study period, we collected field measurements of discharge (in liters per second) during maintenance visits every three weeks at the watershed outlet. We then used these field discharge estimates with corresponding water level measurements from the continuous record to generate a discharge-stage rating curve and estimate continuous discharge.

For further information on pressure transducer data related to this resource, please see: Peterson, D., N. Jones (2026). Talladega Pressure Transducer Data (AIMS_SE_TAL_PRES), HydroShare, http://www.hydroshare.org/resource/93e2861410e647d9a710eea036832dbe

For further information on field discharge measurements related to this resource, please see: Plont, S., D. Peterson, M. Wolford, N. Jones, S. Speir (2025). AIMS Talladega Field Discharge Data (AIMS_SE_TAL_DISL), HydroShare, http://www.hydroshare.org/resource/0e7ad0451bdc45d2b0a51bb538a10909

This resource contains two files:

1) "DISC_SE_TAL_TLM01_ReadME.xlsx": This metadata file contains the relevant site information, the discharge-stage rating curve along with the measurements used to derive it, and information on the various quality assurance-quality check (QAQC) flags used in the continuous discharge time series file.

2)"DISC_SE_TAL_TLM01_20210824_20241015.csv": This data file contains the continuous discharge time series estimated using the discharge-stage rating curve.

ABSTRACT:

This study was conducted in the Shambley Creek research watershed (outlet location: 32.98410915, -88.01334337) on privately owned property in Greene County (AL, USA). The watershed drains a non-perennial unnamed tributary to Shambley Creek, and contains 0.70 km^2 of coniferous forest managed for silviculture in the East Gulf Coastal Plain physiographic section. Located near Eutaw, AL, the watershed spans an elevation range from 63 to 94 m above sea level, and is a tributary to the Sipsey River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 7.3°C and 27.4°C respectively, and mean annual precipitation of 1,350 mm/yr.

These data were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 9 August 2021 and 28 September 2024, we monitored surface water level at the outlet (WHM01) of the AIMS Talladega watershed at 15 minute intervals using a vented pressure transducer (Seametrics PT12 vented pressure/temperature sensor). Over the study period, we collected field measurements of discharge (in liters per second) during maintenance visits every three weeks at the watershed outlet. We then used these field discharge estimates with corresponding water level measurements from the continuous record to generate a discharge-stage rating curve and estimate continuous discharge.

For further information on pressure transducer data related to this resource, please see: Peterson, D., N. Jones (2026). Shambley Creek Pressure Transducer Data (AIMS_SE_WHR_PRES), HydroShare, http://www.hydroshare.org/resource/bc34c8b51c514bf4a6e0a44493bf8ca3

For further information on field discharge measurements related to this resource, please see: Plont, S., D. Peterson, N. Jones, S. Speir (2025). AIMS Shambley Creek Field Discharge Data (AIMS_SE_WHR_DISL), HydroShare, http://www.hydroshare.org/resource/eedcfcb232ee45a6915bd26c68e301e8

This resource contains two files:

1) "DISC_SE_WHR_WHM01_ReadME.xlsx": This metadata file contains the relevant site information, the discharge-stage rating curve along with the measurements used to derive it, and information on the various quality assurance-quality check (QAQC) flags used in the continuous discharge time series file.

2)"DISC_SE_WHR_WHM01_20210809_20240928.csv": This data file contains the continuous discharge time series estimated using the discharge-stage rating curve.

ABSTRACT:

This study was conducted in the Paint Rock research watershed (outlet location: 34.96861724, -86.16501705) on privately owned property in Jackson County (AL, USA). The watershed drains a non-perennial unnamed tributary to Burks Creek, and contains 2.97 km^2 of deciduous forest in the Cumberland Plateau physiographic section. Located near Estillfork, AL, the watershed spans an elevation range from 211 to 550 m above sea level, and is a tributary to the Paint Rock River (within the larger Tennessee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 4.4°C and 25.4°C respectively, and mean annual precipitation of 1,390 mm/yr.

These data were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 27 July 2021 and 3 October 2024, we monitored surface water level at the outlet (PRM01) of the AIMS Talladega watershed at 15 minute intervals using a vented pressure transducer (Seametrics PT12 vented pressure/temperature sensor). Over the study period, we collected field measurements of discharge (in liters per second) during maintenance visits every three weeks at the watershed outlet. We then used these field discharge estimates with corresponding water level measurements from the continuous record to generate a discharge-stage rating curve and estimate continuous discharge.

For further information on pressure transducer data related to this resource, please see: Peterson, D., N. Jones (2026). Paint Rock Pressure Transducer Data (AIMS_SE_PRF_PRES), HydroShare, http://www.hydroshare.org/resource/a45b5e24dafc4a76a665405664afada7

For further information on field discharge measurements related to this resource, please see: Plont, S., D. Peterson, N. Jones, S. Speir (2025). AIMS Paint Rock Field Discharge Data (AIMS_SE_PRF_DISL), HydroShare, http://www.hydroshare.org/resource/d52b989e537349019842dba236627b66

This resource contains two files:

1) "DISC_SE_TAL_PRM01_ReadME.xlsx": This metadata file contains the relevant site information, the discharge-stage rating curve along with the measurements used to derive it, and information on the various quality assurance-quality check (QAQC) flags used in the continuous discharge time series file.

2)"DISC_SE_PRF_PRM01_20210727_20241003.csv": This data file contains the continuous discharge time series estimated using the discharge-stage rating curve.

ABSTRACT:

These data were collected in support of the sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. This study was conducted in the Talladega research watershed (outlet location: 33.76219799, -85.59550775) in the Talladega National Forest (Cleburne County, AL, USA). The watershed drains a non-perennial unnamed tributary of Pendergrass Creek, and contains 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. Located near Anniston, AL, the watershed spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr. Between 7 October 2021 and 4 October 2024, we measured physicochemical parameters including water temperature (degrees Celsius), barometric pressure (mmHg), specific conductance (microSiemens per centimeter), dissolved oxygen, pH, and turbidity at the watershed outlet (TLM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), seasonally at seven distributed, long term monitoring sites (AIMS Approach 2), and during a large synoptic sampling efforts concentrated across the watershed (AIMS Approach 3) using a YSI Pro1030 Waterproof Handheld meter. Readings were allowed to stabilize while water sampling took place. Data is not available when the site was dry - as noted by the flow_state column. In addition, some sampling events lacked a YSI handheld and data is therefore missing.

ABSTRACT:

These data were collected in support of the sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project.This study was conducted in the Shambley Creek research watershed (outlet location: 32.98410915, -88.01334337) on privately owned property in Greene County (AL, USA). The watershed drains a non-perennial unnamed tributary to Shambley Creek, and contains 0.70 km^2 of coniferous forest managed for silviculture in the East Gulf Coastal Plain physiographic section. Located near Eutaw, AL, the watershed spans an elevation range from 63 to 94 m above sea level, and is a tributary to the Sipsey River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 7.3°C and 27.4°C respectively, and mean annual precipitation of 1,350 mm/yr. Between 27 September 2021 and 30 July 2024, we measured physicochemical parameters including water temperature (degrees Celsius), barometric pressure (mmHg), specific conductance (microSiemens per centimeter), dissolved oxygen, pH, and turbidity at the watershed outlet (WHM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), seasonally at seven distributed, long term monitoring sites across the watershed (AIMS Approach 2) using a YSI Pro1030 Waterproof Handheld meter. Readings were allowed to stabilize while water sampling took place. Data is not available when the site was dry - as noted by the flow_state column. In addition, some sampling events lacked a YSI handheld and data is therefore missing.

ABSTRACT:

These samples were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. This study was conducted in the Paint Rock research watershed (outlet location: 34.96861724, -86.16501705) on privately owned property in Jackson County (AL, USA). The watershed drains a non-perennial unnamed tributary to Burks Creek, and contains 2.97 km^2 of deciduous forest in the Cumberland Plateau physiographic section. Located near Estillfork, AL, the watershed spans an elevation range from 211 to 550 m above sea level, and is a tributary to the Paint Rock River (within the larger Tennessee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 4.4°C and 25.4°C respectively, and mean annual precipitation of 1,390 mm/yr. Between 8 September 2021 and 1 August 2024, we measured physicochemical parameters including water temperature (degrees Celsius), barometric pressure (mmHg), specific conductance (microSiemens per centimeter), dissolved oxygen, pH, and turbidity at the watershed outlet (PRM01) every 3 weeks during routine sensor maintenance (AIMS Approach 1), seasonally at seven distributed, long term monitoring sites across the watershed (AIMS Approach 2) using a YSI Pro1030 Waterproof Handheld meter. Readings were allowed to stabilize while water sampling took place. Data is not available when the site was dry - as noted by the flow_state column. In addition, some sampling events lacked a YSI handheld and data is therefore missing.

ABSTRACT:

This study was conducted in the Talladega research watershed (outlet location: 33.76219799, -85.59550775) in the Talladega National Forest (Cleburne County, AL, USA). The watershed drains a non-perennial unnamed tributary of Pendergrass Creek, and contains 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. Located near Anniston, AL, the watershed spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr.

These data were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 15 September 2021 and 26 September 2023, we also deployed a s::can spectro::lyser V3 UV-Vis spectrophotometer (Messtechnik GmbH, Vienna, Austria) to measure light absorbance at wavelengths from 190 to 750 nm at 2.5 nm intervals every 15 minutes. These absorbance spectra were turbidity-compensated using a s::can proprietary algorithm. Due to the complexity of the absorbance spectra time series generated by the submersible UV-Vis spectrophotometer, we used a simplified data quality assurance method to filter out erroneous absorbance spectra. We used error codes recorded on the instrument, field notes, and water-level and turbidity data from the watershed outlet to help remove all spectra during periods of instrument malfunction, burial, and desiccation. We further filtered the absorbance data time series by first selecting three wavelengths (200 nm, 255 nm, 400 nm) to be representative of the entire UV-VIS absorbance spectra. We removed entire recorded spectra when 1) absorbance values at a given time point were negative at 200 or 255 nm and 2) when absorbance values at a given time point exceeded three standard deviations of the rolling mean absorbance values at 200, 255, or 400 nm.

ABSTRACT:

This resource specifically includes the greenhouse gas (GHGS) seasonal (approach 2) and synoptic (approach 3) sample datasets collected in our Talladega research watershed, including carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O). Approach 2 and approach 3 sampling campaigns conducted in Talladega research watershed were designed in support of the sampling goals of the NSF funded Aquatic Intermittency effects on Microbiomes in Streams (AIMS) project (EPSCoR funded project, OIA 2019603). Specifically, the MIMS dissolved gas data was collected by the biogeochemistry team to better understand the effects of stream drying on dissolved gases in the southeastern US. The Talladega National Forest (Cleburne County, AL, USA; Watershed Outlet Lat/Long: 33.76219799, -85.59550775) contains a non-perennial unnamed tributary of Pendergrass Creek, where our study sites were located, as well as 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. The research watershed also spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr.

On six different occasions from June 29, 2022 to January 29, 2024, we collected dissolved gas (GHGS) samples seasonally across 7 sampling locations (approach 2). On one separate occasion during June 2022, we collected GHGS samples from 49 sampling locations (approach 3) within the same Talladega research watershed. For the approach 3 sampling campaign, the sites were selected to balance multiple competing priorities: (i) strategically targeting existing monitoring infrastructure with long-term data; (ii) including sites near several known springs and tributary junctions; and (iii) including a range of drainage area and topographic wetness index (TWI) values, both of which have been correlated with flow permanence. Briefly, the sites selected based on drainage area and TWI were chosen by binning drainage area into 10 bins and then binning TWI into quintiles within each drainage area bin. We then randomly selected a point in each bin after accounting for points selected based on existing infrastructure, springs, and tributaries, as well as enforcing a minimum spacing of 100 m between locations. We then made minor adjustments to points to account for field conditions, for instance adjusting locations with respect to a road crossing.

Included in the Content section is the sampling datasheet that was used during field collection, as well as one compiled excel file containing approach 2 and 3 GHGS TAL data. Within the compiled excel file, the 'Working Files' tab contains the processed GHGS data before taking averages of all the replicates. The 'Final Data' tab has the data that should be used for analyses with the averages of all 3 replicates and standard deviations (sd) in uM units. Also, there are columns regarding whether the sample was from approach 2 or 3. Approach 1 samples were any MIMS samples collected at Talladega watershed outlet site (TLMO1) during approach 2 or 3 sampling campaigns.

ABSTRACT:

This resource specifically includes the Membrane Inlet Mass Spectrometer (MIMS) dissolved gas seasonal (approach 2) and synoptic (approach 3) sample datasets collected in our Talladega research watershed, including Argon (Ar), Oxygen (O2), Nitrogen (N2), N2:Ar ratio, and O2:Ar ratio. Approach 2 and approach 3 sampling campaigns conducted in Talladega research watershed were designed in support of the sampling goals of the NSF funded Aquatic Intermittency effects on Microbiomes in Streams (AIMS) project (EPSCoR funded project, OIA 2019603). Specifically, the MIMS dissolved gas data was collected by the biogeochemistry team to better understand the effects of stream drying on dissolved gases in the southeastern US. The Talladega National Forest (Cleburne County, AL, USA; Watershed Outlet Lat/Long: 33.76219799, -85.59550775) contains a non-perennial unnamed tributary of Pendergrass Creek, where our study sites were located, as well as 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. The research watershed also spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr.

On six different occasions from March 29, 2022 to January 29, 2024, we collected dissolved gas (MIMS) samples seasonally across 7 sampling locations (approach 2). On one separate occasion during June 2022, we collected MIMS samples from 49 sampling locations (approach 3) within the same Talladega research watershed. For the approach 3 sampling campaign, the sites were selected to balance multiple competing priorities: (i) strategically targeting existing monitoring infrastructure with long-term data; (ii) including sites near several known springs and tributary junctions; and (iii) including a range of drainage area and topographic wetness index (TWI) values, both of which have been correlated with flow permanence. Briefly, the sites selected based on drainage area and TWI were chosen by binning drainage area into 10 bins and then binning TWI into quintiles within each drainage area bin. We then randomly selected a point in each bin after accounting for points selected based on existing infrastructure, springs, and tributaries, as well as enforcing a minimum spacing of 100 m between locations. We then made minor adjustments to points to account for field conditions, for instance adjusting locations with respect to a road crossing.

Included in the Content section is the sampling datasheet that was used during field collection, as well as one compiled excel file containing approach 2 and 3 MIMS TAL data. Within the compiled excel file, the 'Working Files' tab contains the processed MIMS data before taking averages of all the replicates. The 'Final Data' tab has the data that should be used for analyses with the averages of all 3 replicates and standard deviations (sd) in uM units. Also, there are columns regarding whether the sample was from approach 2 or 3. Approach 1 samples were any MIMS samples collected at Talladega watershed outlet site (TLMO1) during approach 2 or 3 sampling campaigns. The raw MIMS data was processed and calculated via the package 'MIMSY' (R Package; M. Kelly (2020), https://cran.r-project.org/package=mimsy) and has 3 data points per replicate.

ABSTRACT:

This study was conducted in the Shambley Creek research watershed (outlet location: 32.98410915, -88.01334337) on privately owned property in Greene County (AL, USA). The watershed drains a non-perennial unnamed tributary to Shambley Creek, and contains 0.70 km^2 of coniferous forest managed for silviculture in the East Gulf Coastal Plain physiographic section. Located near Eutaw, AL, the watershed spans an elevation range from 63 to 94 m above sea level, and is a tributary to the Sipsey River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 7.3°C and 27.4°C respectively, and mean annual precipitation of 1,350 mm/yr.

This resource specifically includes the Membrane Inlet Mass Spectrometer (MIMS) dissolved gas seasonal (approach 2) and synoptic (approach 3) sample datasets collected in our Shambley Creek research watershed, including Argon (Ar), Oxygen (O2), Nitrogen (N2), N2:Ar ratio, and O2:Ar ratio. We collected gas MIMS samples on six different occasions from March 24, 2022 to January 22, 2024 at seven distributed sites (approach 2). The final data tab includes averaged data for N2:Ar (unitless), O2:Ar (unitless), N2 (uM), O2 (uM), and Ar (uM), with standard deviations. Average NAs indicate MIMS dissolved gas samples were not run or that samples were missing.

ABSTRACT:

This study was conducted in the Paint Rock research watershed (outlet location: 34.96861724, -86.16501705) on privately owned property in Jackson County (AL, USA). The watershed drains a non-perennial unnamed tributary to Burks Creek, and contains 2.97 km^2 of deciduous forest in the Cumberland Plateau physiographic section. Located near Estillfork, AL, the watershed spans an elevation range from 211 to 550 m above sea level, and is a tributary to the Paint Rock River (within the larger Tennessee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 4.4°C and 25.4°C respectively, and mean annual precipitation of 1,390 mm/yr.

This resource specifically includes the Membrane Inlet Mass Spectrometer (MIMS) dissolved gas seasonal (approach 2) sample dataset, including Argon (Ar), Oxygen (O2), Nitrogen (N2), N2:Ar ratio, and O2:Ar ratio. We collected seasonal MIMS samples at 7 sites throughout the Paint Rock watershed on six different occasions from March 13, 2022 to February 19, 2024.

ABSTRACT:

This resource contains high-frequency EXOS water quality sensor data collected at the outlet of the Talladega study watershed, including conductivity, dissolved oxygen, turbidity, fluorescent dissolved organic matter concentrations, and temperature. This study was conducted in the Talladega research watershed (outlet location: 33.76219799, -85.59550775) in the Talladega National Forest (Cleburne County, AL, USA). The watershed drains a non-perennial unnamed tributary of Pendergrass Creek, and contains 0.92 km^2 of mixed coniferous and deciduous forest in the Piedmont Upland physiographic section. Located near Anniston, AL, the watershed spans an elevation range from 345 to 456 m above sea level and is a tributary to the Coosa River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 5.3°C and 25.3°C respectively, and mean annual precipitation of 1,400 mm/yr.

These data were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 15 September 2021 and 31 December 2024, we monitored surface water quality and physicochemistry at the outlet (TLM01) of the AIMS Talladega watershed at 15 minute intervals using a multi-parameter sonde (YSI EXO2).

Included in this excel file are four tabs: 1. Metadata: methods, authorship and site information; 2. Data Types: column descriptions for each data file in this resource; 3. Site_info; site name, latitude and longitude for the site at which the sensor was deployed; 4. QAQC'ed EXO data

Sensors were maintained every three weeks according to this SOP: Flynn, S., S. Godsey, R. Hale, R. Lanfear, E. Seybold, S. Speir, M. Wolford (2025). Sensor Maintenance SOP, HydroShare, http://www.hydroshare.org/resource/f056a431a6794d2dbf9f6206c00ac560

Sensors were calibrated quarterly according to this SOP: Flynn, S., R. Lanfear, E. Seybold (2025). Sensor Calibration SOP, HydroShare, http://www.hydroshare.org/resource/85fa713daf0142a4b45e3bc8ff1d1e30

FDOM was corrected for Turbidity and Temperature according to the methods from Downing et al 2012.

For information about installing sensors, please see https://www.hydroshare.org/resource/703cf05242f7455c8dfb072dd072c962/

For further information on pressure transducer data related to this resource, please see: Peterson, D., N. Jones (2025). Talladega Pressure Transducer Data (AIMS_SE_TAL_approach1_PRES), HydroShare, http://www.hydroshare.org/resource/93e2861410e647d9a710eea036832dbe

For further information on continuous discharge measurements at the watershed outlet/related to this resource, please see: Plont, S., M. Wolford, K. Zarek, D. Peterson, N. Jones, S. Speir (2025). AIMS Talladega Continuous Discharge at Watershed Outlet Data (AIMS_SE_TAL_DISC), HydroShare, http://www.hydroshare.org/resource/fc7ae2d28e3c481d805902a79af90a95

For further information on field discharge measurements related to this resource, please see: Plont, S., D. Peterson, M. Wolford, N. Jones, S. Speir (2025). AIMS Talladega Field Discharge Data (AIMS_SE_TAL_DISL), HydroShare, http://www.hydroshare.org/resource/0e7ad0451bdc45d2b0a51bb538a10909

ABSTRACT:

This resource contains high-frequency EXOS water quality sensor data collected at the outlet of the Paint Rock study watershed, including conductivity, dissolved oxygen, turbidity, fluorescent dissolved organic matter concentrations, and temperature. This study was conducted in the Paint Rock research watershed (outlet location: 34.96861724, -86.16501705) on privately owned property in Jackson County (AL, USA). The watershed drains a non-perennial unnamed tributary to Burks Creek, and contains 2.97 km^2 of deciduous forest in the Cumberland Plateau physiographic section. Located near Estillfork, AL, the watershed spans an elevation range from 211 to 550 m above sea level, and is a tributary to the Paint Rock River (within the larger Tennessee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 4.4°C and 25.4°C respectively, and mean annual precipitation of 1,390 mm/yr.

These data were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 1 August 2021 and 4 September 2024, we monitored surface water quality and physicochemistry at the outlet (PRM01) of the AIMS Paint Rock watershed at 15 minute intervals using a multi-parameter sonde (YSI EXO2).

Included in this excel file are four tabs: 1. Metadata: methods, authorship and site information; 2. Data Types: column descriptions for each data file in this resource; 3. Site_info; site name, latitude and longitude for the site at which the sensor was deployed; 4. QAQC'ed EXO data

Sensors were maintained every three weeks according to this SOP: Flynn, S., S. Godsey, R. Hale, R. Lanfear, E. Seybold, S. Speir, M. Wolford (2025). Sensor Maintenance SOP, HydroShare, http://www.hydroshare.org/resource/f056a431a6794d2dbf9f6206c00ac560

Sensors were calibrated quarterly according to this SOP: Flynn, S., R. Lanfear, E. Seybold (2025). Sensor Calibration SOP, HydroShare, http://www.hydroshare.org/resource/85fa713daf0142a4b45e3bc8ff1d1e30

FDOM was corrected for Turbidity and Temperature according to the methods from Downing et al 2012.

For information about installing sensors, please see https://www.hydroshare.org/resource/703cf05242f7455c8dfb072dd072c962/

For further information on pressure transducer data related to this resource, please see: Peterson, D., N. Jones (2025). Paint Rock Pressure Transducer Data (AIMS_SE_PRF_approach1_PRES), HydroShare, http://www.hydroshare.org/resource/a45b5e24dafc4a76a665405664afada7

For further information on continuous discharge measurements at the watershed outlet/related to this resource, please see: Plont, S., S. Speir, D. Peterson, N. Jones (2025). AIMS Paint Rock Continuous Discharge at Watershed Outlet Data (AIMS_SE_PRF_DISC), HydroShare, http://www.hydroshare.org/resource/043fc07f0c3b47bcabbd0bf5600d929f

For further information on field discharge measurements related to this resource, please see: Plont, S., D. Peterson, N. Jones, S. Speir (2025). AIMS Paint Rock Field Discharge Data (AIMS_SE_PRF_DISL), HydroShare, http://www.hydroshare.org/resource/d52b989e537349019842dba236627b66

ABSTRACT:

This resource contains high-frequency EXOS water quality sensor data collected at the outlet of the Talladega study watershed, including conductivity, dissolved oxygen, turbidity, fluorescent dissolved organic matter concentrations, and temperature. This study was conducted in the Shambley Creek research watershed (outlet location: 32.98410915, -88.01334337) on privately owned property in Greene County (AL, USA). The watershed drains a non-perennial unnamed tributary to Shambley Creek, and contains 0.70 km^2 of coniferous forest managed for silviculture in the East Gulf Coastal Plain physiographic section. Located near Eutaw, AL, the watershed spans an elevation range from 63 to 94 m above sea level, and is a tributary to the Sipsey River (within the larger Mobile-Tombigbee basin). The region has a humid subtropical climate, with mean daily January and July air temperatures of 7.3°C and 27.4°C respectively, and mean annual precipitation of 1,350 mm/yr.

These data were collected in support of the core sampling goals of the Aquatic Intermittency effects on Microbiomes in Streams (AIMS) Project. Between 1 August 2021 and 1 August 2024, we monitored surface water quality and physicochemistry at the outlet (WHM01) of the AIMS Shambley Creek watershed at 15 minute intervals using a multi-parameter sonde (YSI EXO2).

Included in this excel file are four tabs: 1. Metadata: methods, authorship and site information; 2. Data Types: column descriptions for each data file in this resource; 3. Site_info; site name, latitude and longitude for the site at which the sensor was deployed; 4. QAQC'ed EXO data

Sensors were maintained every three weeks according to this SOP: Flynn, S., S. Godsey, R. Hale, R. Lanfear, E. Seybold, S. Speir, M. Wolford (2025). Sensor Maintenance SOP, HydroShare, http://www.hydroshare.org/resource/f056a431a6794d2dbf9f6206c00ac560

Sensors were calibrated quarterly according to this SOP: Flynn, S., R. Lanfear, E. Seybold (2025). Sensor Calibration SOP, HydroShare, http://www.hydroshare.org/resource/85fa713daf0142a4b45e3bc8ff1d1e30

FDOM was corrected for Turbidity and Temperature according to the methods from Downing et al 2012.

For information about installing sensors, please see https://www.hydroshare.org/resource/703cf05242f7455c8dfb072dd072c962/

For further information on pressure transducer data related to this resource, please see: Peterson, D., N. Jones (2025). Shambley Creek Pressure Transducer Data (AIMS_SE_WHR_approach1_PRES), HydroShare, http://www.hydroshare.org/resource/bc34c8b51c514bf4a6e0a44493bf8ca3

For further information on continuous discharge measurements at the watershed outlet/related to this resource, please see: Plont, S., S. Speir, D. Peterson, N. Jones (2025). AIMS Shambley Creek Continuous Discharge at Watershed Outlet Data (AIMS_SE_WHR_DISC), HydroShare, http://www.hydroshare.org/resource/535797126b134ceaab9838df0ca00885

For further information on field discharge measurements related to this resource, please see: Plont, S., D. Peterson, N. Jones, S. Speir (2025). AIMS Shambley Creek Field Discharge Data (AIMS_SE_WHR_DISL), HydroShare, http://www.hydroshare.org/resource/eedcfcb232ee45a6915bd26c68e301e8