Shannon L Speir

University of Notre Dame | PhD Student

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ABSTRACT:

We collected intact sediment cores from the Sunflower River (near Ruleville, MS), and used flow-through incubations to test the interaction of nitrate concentration, carbon availability, and temperature on nitrous oxide production and yields (as % of total denitrification). We conducted the sediment core incubations at both 15 °C and 25 °C. Cores were dosed with a range of nitrate concentrations (1-6 mg L-1) and two carbon amendments: ambient (0.5 mg L-1) and +carbon (13.5 mg L-1). We used membrane inlet mass spectrometry (MIMS) to measure nitrous oxide, dinitrogen gas, and oxygen concentrations. We used the cadmium reduction method on a Lachat Quickchem Analyzer to measure nitrate concentrations and used the combustion catalytic oxidation method on a Shimadzu TOC-L to measure DOC concentrations.

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Dissolved gas and nutrient data from flow-through sediment core incubations conducted at two temperatures
Created: March 18, 2022, 5:41 p.m.
Authors: Speir, Shannon L · Jennifer L Tank · Jason M Taylor · Amelia L Grose

ABSTRACT:

We collected intact sediment cores from the Sunflower River (near Ruleville, MS), and used flow-through incubations to test the interaction of nitrate concentration, carbon availability, and temperature on nitrous oxide production and yields (as % of total denitrification). We conducted the sediment core incubations at both 15 °C and 25 °C. Cores were dosed with a range of nitrate concentrations (1-6 mg L-1) and two carbon amendments: ambient (0.5 mg L-1) and +carbon (13.5 mg L-1). We used membrane inlet mass spectrometry (MIMS) to measure nitrous oxide, dinitrogen gas, and oxygen concentrations. We used the cadmium reduction method on a Lachat Quickchem Analyzer to measure nitrate concentrations and used the combustion catalytic oxidation method on a Shimadzu TOC-L to measure DOC concentrations.

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